Other

Part:BBa_I716331:Design

Designed by: nhu nguyen   Group: iGEM07_Berkeley_UC   (2007-07-02)

ThpA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 29
    Illegal PstI site found at 590
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 29
    Illegal PstI site found at 590
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 29
    Illegal suffix found in sequence at 575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 29
    Illegal PstI site found at 590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 29
    Illegal PstI site found at 590
    Illegal AgeI site found at 1060
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 496


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

PCR ThpA F/R on ThpA (553bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BglII/XhoI, 2054+910, L)
Product is pBca9145-BBa I716331



ThpAF Forward BglII site anneals to ThpA cgtacAGATCTatgaccgccgcaccagcagattttgcccgtgcccgaagcgaGttcctcagtatcg

ThpAR Reverse XhoI site anneals to ThpA anneals to XhoI cgcatctcgagtcaggtggcgaacgggccta


References